Table 1. Uptake of different metal ions and complementation of SMF1 null mutants by DCT1 and mutants of loop I.
| Mutant | Fe uptake | Mn uptake | Co uptake | Complementation |
|---|---|---|---|---|
| DCT1 | 18.0 ± 1.8 | 45.4 ± 11.1 | 66.2 ± 7.3 | + |
| G119A | <0.2 | 0 | 0 | - |
| G119C | 0 | |||
| G119V | 0 | |||
| E122A | 7.3 ± 2.2 | 9 ± 0.8 | 16.4 ± 4.5 | - |
| D124A | 3.8 ± 0.3 | 0.7 ± 0.1 | 1.7 ± 0.3 | - |
| Q126D | <0.1 | 0 | 0 | - |
| D124A + Q126D | 3.26 ± 0 | 0.6 ± 0.2 | 2.2 ± 0.1 | - |
| G119A + D124A + Q126D | 0 | 0 | 0 | - |
| K134S | 2.3 ± 0.3 | 5.41 ± 0.5 | 9.3 ± 0.7 | + |
Representative experiments of oocytes obtained from at least three different frogs are shown. The results and standard deviations are expressed as metal uptake of pmol per oocyte per h. The yeast complementation assay was performed with smf1Δ mutant as described in Materials and Methods.