Figure 2.

Interaction of Axin and GSK-3β in vivo. SW480 cells were transfected with pcDNA3.1 vector alone or the same vector containing FL-Axin or ΔN-Axin (7 μg each, lanes 1, 2, and 3 respectively). Cell lysates were immunoprecipitated and analyzed by Western blotting as indicated. Similar results were obtained from other cell lines, including 293 cells, Chinese hamster ovary cells, and COS7 cells.