Figure 5.

Inhibition of Lef-1 reporter activity by Axin. The assays were performed as duplicate transfections. Representative data from three independent experiments are shown. Luciferase activity was normalized to transfection efficiency by using β-gal activity. There were no significant differences in β-gal activity in each transfection. Luciferase and β-gal activity were measured 48 h after transfection. (Bars = SEM.) (A) 293 cells were seeded and transfected in 12-well culture plates. The Wnt-1 gene (0.075 μg) was transfected. (B) SW480 cells were seeded and transfected in 12-well culture plates. The inhibition of the Lef-1 reporter gene was constantly higher in ΔN-Axin-transfected cells than in FL-Axin-transfected cells. This may depend on the difference of protein expression level (Fig. 2).