Skip to main content
. 1998 Mar 17;95(6):3024–3029. doi: 10.1073/pnas.95.6.3024

Figure 2.

Figure 2

Pharmacological characterization of quail EDNRB2 receptor expressed in transfected Ltk− cells. (A and B) Competitive radioligand-binding assay. Displacement of specific [125I]EDN 1 binding to cells expressing quail EDNRB2 (A) and human EDNRB (B) by increasing concentrations of unlabeled endothelin-related peptides are determined in quadruplicates. Values are plotted as percentage of the levels of binding without competitors. (C and D) Dose–response relationships of intracellular Ca2+ transients evoked by endothelin-related peptides in cells expressing quail EDNRB2 (C) and human EDNRB (D). Peak cytoplasmic Ca2+ concentrations are determined in quadruplicates and plotted as percentage of responses evoked by 10−5 M noradrenaline.