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. 2003 Sep 5;100(19):10830–10835. doi: 10.1073/pnas.1332668100

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Copyright © 2003, The National Academy of Sciences

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Fig. 1. — Schematic representation of virus platings as carried out in this article. (A) Serial transfers. Clones , and (described in ref. 19 and in Materials and Methods) were diluted and plated for isolation of virus from an individual plaque (upper plates) and for titration of infectious particles (lower plates). Each viral population was derived from a plaque of the previous plating, and the serial plaque-to-plaque transfers were repeated a total of 50 times. (B) Control platings. C-S8c1, p50, MARLS, and RGG were repeatedly plated to determine the influence of the cells on virus titer. Each titration was a dead end for these control platings of nonevolving virus. A total of 50 platings were carried out with aliquots of the same population (indicated as 1). Procedures for virus plating and plaque development are detailed in Materials and Methods.

Inline graphic — Schematic representation of virus platings as carried out in this article. (A) Serial transfers. Clones , and (described in ref. 19 and in Materials and Methods) were diluted and plated for isolation of virus from an individual plaque (upper plates) and for titration of infectious particles (lower plates). Each viral population was derived from a plaque of the previous plating, and the serial plaque-to-plaque transfers were repeated a total of 50 times. (B) Control platings. C-S8c1, p50, MARLS, and RGG were repeatedly plated to determine the influence of the cells on virus titer. Each titration was a dead end for these control platings of nonevolving virus. A total of 50 platings were carried out with aliquots of the same population (indicated as 1). Procedures for virus plating and plaque development are detailed in Materials and Methods.