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. 2003 Sep 8;100(19):10854–10859. doi: 10.1073/pnas.1934561100

Fig. 3.

Fig. 3.

Analysis of T-TFs in strains with mutations affecting DNA damage checkpoints (ddc1, mec3, and pds1) or nonhomologous DNA end-joining (yku70, yku80, and lig4). (a) Effect of mutations in pds1, ddc1, mec3, yku70, and yku80 on T-TF formation. Strains of the ddc1 tel1, mec3 tel1, pds1 tel1,or mec1 tel1 genotypes have the 350-bp PCR fragment diagnostic of T-TFs involving short telomeres, whereas mec1–21 tel1 yku70 and mec1–21 tel1 yku80 strains have multiple larger PCR fragments diagnostic of T-TFs involving longer telomeres. Strain names in lanes 2–12 are: PM197-1a, PM208 (lanes 3 and 4), PM197-7b, PM203-17c, PM195-11d, PM207 (lanes 8 and 9), PM206 (lanes 10 and 11), and PM197-8d. (b) Effect of the lig4 mutation on T-TF formation in association with tel1 and/or mec1 mutations. Strain names in lanes 1–10 are: PM183-1a, PM183-2c, PM183-6b, PM183-2a, PM183-1b (lanes 5 and 6), PM183-3d, PM183-12a, and PM183-13c (lanes 9 and 10).