Table 2.

Rates of forward mutation and deletion formation at the CAN1 locus in strains with mutations affecting genome stability

Genotype	Mutation rate (95% confidence limits)†	Rate of deletions‡
Wild-type*	2.4 × 10-7 (1.8-3.6 × 10-7)	<1 × 10-9
mec1-21*	3.1 × 10-7 (2.5-3.9 × 10-7)	9.5 × 10-8
te/1*	3.4 × 10-7 (2.7-4.8 × 10-7)	5.1 × 10-8
mec1-21 te/1	1.0 × 10-5 (0.6-1.4 × 10-5)	4.6 × 10-6
mec1-21 te/1 + pVL1107	8 × 10-7 (2.7-4.8 × 10-7)	2.4 × 10-7
t/c1*	2.0 × 10-6 (0.7-4.5 × 10-6)	5.6 × 10-8
t/c1§	1.3 × 10-6 (1.2-2 × 10-6)	4 × 10-7
mec1-21 t/c1	2.8 × 10-6 (1.8-4.7 × 10-6)	3.1 × 10-7
mec3 te/1	9.0 × 10-6 (3.2-16 × 10-6)	5.4 × 10-6
ddc1 te/1	7.5 × 10-6 (3.2-12 × 10-6)	3.5 × 10-6
pds1 te/1	5.2 × 10-6 (2.8-13 × 10-6)	3.3 × 10-7
mec1-21 lig4	2.5 × 10-7 (1.5-3.2 × 10-7)	<1 × 10-9
te/1 lig4	3.6 × 10-7 (3.1-4.4 × 10-7)	1.8 × 10-8
mec1-21 te/1 lig4	1.8 × 10-6 (0.8-5.7 × 10-6)	4.4 × 10-7
te/1 yku80	4 × 10-7 (3.6-7 × 10-7)	2 × 10-8
mec1-21 yku80	5 × 10-7 (3.6-5.7 × 10-7)	2.5 × 10-8
mec1-21 te/1 yku80	1.5 × 10-6 (0.8-2.9 × 10-6)	5.3 × 10-7
te/1 yku70	8.6 × 10-7 (4.9-12.3 × 10-7)	<1 × 10-9
mec1-21 yku70	4.1 × 10-7 (2.8-6.3 × 10-7)	2.1 × 10-8
mec1-21 te/1 yku70	1.4 × 10-6 (0.6-3.2 × 10-6)	2.8 × 10-7

Table 5, which is published as supporting information on the PNAS web site, is an extended form of this table.

^*Data from ref. 7.

^†For each strain, we measured the frequency of canavanine-resistant cells in 20 independent cultures. Rate calculations were done as described (12).

^‡The rate of deletions was calculated by multiplying the forward rate of mutations at the CAN1 locus by the fraction of these mutations that were deletions (determined by PCR analysis of 20 independent can1 mutants).

^§The rate of deletions for this strain was calculated by a method different from that used for the other strains (details are in Table 5).