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. 2003 Sep 5;100(19):10890–10895. doi: 10.1073/pnas.1832775100

Fig. 3.

Fig. 3.

Effect of TNF-α and IL-1β pretreatment on NF-κB-dependent restriction of RSV replication and cytopathogenicity. (A) Plaque assay analysis of medium supernatants from A549 cells pretreated with 20 ng/ml TNF-α or IL-1β for 8–16 h before RSV infection. (B) Plaque assay analysis of culture supernatants from IκB-SR or GFP expressing A549 cells, pretreated with either TNF-α or IL-1β for 16 h before RSV infection. (C) Expression of transfected NF-κB-Luc in A549 cells treated with either TNF-α or IL-1β (20 ng/ml for 2 h) in the presence of IκB-SR or control GFP. (D) Plaque assay analysis of medium supernatants from A549 cells pretreated with 20 ng/ml TNF-α or IL-1β for 24 h before HPIV-3 infection.