ASCT activity in homogenates of L. infantum promastigotes. ASCT activity was determined in homogenates of L. infantum promastigotes, containing 12, 23, or 46 μg of protein, as described in Materials and Methods. The assay mixture contained 10 mM succinate, 2 mM ADP, and 10 mM Pi. (Inset) slopes of the graphs of acetate production vs. time are plotted against the amount of homogenate. One example of two independent experiments is shown.