Figure 6.

The dependence of the ASCT activity on ADP and P_i in the presence of 1 mM succinate. ASCT activity was determined as described in Materials and Methods in the presence of 1 mM succinate. Open bars represent ASCT activity (acetate production) in the presence of ADP (2 mM) and P_i (10 mM), whereas closed bars represent enzyme activity in the absence of ADP and P_i. Shown are the means ± SD of three independent experiments; ∗ indicates significantly different from the ASCT activity in the presence of ADP and P_i. P < 0.02.