Table 2.
Partial purification of acetate:succinate CoA transferase from L. mexicana mexicana promastigotes
| Fraction | Volume, ml | Protein, mg | Specific activity ASCT, nmol/min·mg | Purification factor | Recovery, % |
|---|---|---|---|---|---|
| Homogenate | 0.85 | 4.80 | 156.1 | 1 | 100 |
| Supernatant | 0.80 | 2.64 | 284.9 | 1.8 | 100 |
| Superose 6 | 11.0 | 0.91 | 200.8 | 1.3 | 24 |
| Mono Q | 3.2 | 0.1 | 873.2 | 5.6 | 12 |
Acetate:succinate CoA transferase from L. mexicana mexicana promastigotes partially was purified as described in Materials and Methods. One example of two independent experiments is shown. ASCT, acetate:succinate CoA transferase.