Fig. 1.
Immunization with SEREX-defined wild-type antigens enhances pulmonary metastasis. BALB/c mice were injected with 1 × 106 CMS5m (a) or 1 × 105 Colon 26 (b) via the lateral tail vein. Biweekly immunization with plasmids encoding the indicated antigens by using a gene gun commenced 5 days after tumor challenge. Pulmonary metastatic nodules were counted with a dissecting microscope 28 (a) or 21 (b) days after tumor challenge. Data are mean ± SD; n = 5. These experiments were repeated three times with similar results.