Fig. 3.

Enhancement of pulmonary metastasis by transfer of CD4⁺ CD25⁺ T cells from mice immunized with SEREX-defined wild-type antigen. Purified 1 × 10⁵ CD4⁺ CD25⁺ T cells or CD4⁺ CD25^– T cells derived from BALB/c mice (naive, Dna J-like 2, or control vector immunized) were transferred into naive BALB/c mice 7 days before 1 × 10⁶ CMS5m inoculation. Mice were killed 28 days after tumor challenge, and pulmonary metastatic nodules were counted with a dissecting microscope. Data are mean ± SD; n = 5. These experiments were repeated three times with similar results.