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. 2003 Sep 2;100(19):10931–10936. doi: 10.1073/pnas.1932980100

Fig. 2.

Fig. 2.

Expression of DCD in normal and cancerous tissues. (A) Northern blot analyses of normal breast organoids, breast cancer cell lines, primary breast carcinomas, and corresponding normal breast tissue. High DCD expression is detected in only two tumors (15 and 236). The blot was rehybridized with β-actin to indicate equal loading. (B) RT-PCR analyses of breast cancer cell lines using DCD- and β-actin-specific primers. (C) mRNA in situ hybridization using digitonin-labeled DCD riboprobes on tissue sections (tumor and normal 236, red staining; tumors 15 and 238, black staining). Adjacent section stained with hematoxylin/eosin. (D) DCD immunostaining of normal breast tissue, ductal carcinoma in situ, a DCD-positive invasive breast carcinoma (IDC), and sweat gland of the skin. (E) Immunoblot analysis of human sweat and cells transfected with empty or DCD-expressing vector. An ≈11-kDa protein is detected in both transfected cells and sweat.