Fig. 2.

(Upper) Localization and sequencing of the GPR54 gene. Markers used in the genotyping and the recombination point in patient III.4 are indicated. (Lower) DNA sequences from unaffected sib III.1 and affected patient III.2 are shown. The primers used for amplification of exon 5 were localized within intron 4 and in the 3′ untranslated region. The cDNA numbering was used to designate nucleotides in exon 5. In intron 4, nucleotides were numbered starting from the end of the intron. Residues of the 3′ end of intron 4 present in both individuals are underlined.