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E. coli strains |
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| DH10β |
Host for E. coli cloning; F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 Δ(codB-lacI)3 deoR recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ-rpsL(StrR)
|
Life Technology |
| UQ950 |
E. coli DH5α λ(pir) host for cloning; F-Δ(argF-lac)169 Φ80dlacZ58(ΔM15) glnV44(AS) rfbD1 gyrA96(NalR) recA1 endA1 spoT1 thi-1 hsdR17 deoR λpir+ |
D. Lies, Caltech |
| WM3064 |
Donor strain for conjugation: thrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567 ΔdapA1341::[erm pir(wt)] |
W. Metcalf, Univ. of Illinois, Urbana |
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Shewanella strains |
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| ANA-3 |
Isolated from an As-treated wooden pier pilling in a brackish estuary (Eel Pond, Woods Hole, MA); Contains arsDABC; respires on As(V); resistant to > 5 mM arsenite. |
8 |
| ARRA3 |
Shewanella sp. str. ANA-3, ΔarrA3; does not respire As(V) |
This study |
| ARRB1 |
Shewanella sp. str. ANA-3, ΔarrB1; does not respire As(V) |
This study |
| Plasmids/vectors |
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| pSALT1 |
pLAFR5-based 45 kb As(III)r cosmid from ANA-3 genomic DNA library; contains arsDABC, Tcr, confers resistance to As(III) and As(V). |
8 |
| pSMV10 |
9.1-kb mobilizable suicide vector; oriR6K, mobRP4, sacB, Kmr Gmr
|
D. Lies, Caltech |
| pΔarrA3
|
2-kb fusion PCR fragment containing ΔarrA cloned into the Spel site of pSMV10; used to make the ARRA3 ΔarrA strain. |
This study |
| pΔarrBI
|
2-kb fusion PCR fragment containing ΔarrB cloned into the Spel site of pSMV10; used to make the ARRBI ΔarrB strain. |
This study |
| pBBR1MCS-2 |
5.1-kb broad-host range plasmid: Kmr, lacZ
|
15 |
| parrA4 |
arrA4 PCR fragment, including the promoter region, cloned into the Spel site of pBBR1MCS-2 |
This study |
| parrB1 |
arrB1 PCR fragment cloned into the Xhol/Spel sites of pBBR1MCS-2 |
This study |
