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Tgl stimulation of S-motility on 1.0 and 0.5% agar. Nonmotile DK8601 (aglB1 tgl+ ΔpilA) donor cells were mixed with nonmotile DK8602 (aglB1 Δtgl pilA+) recipient cells at a ratio of 1:1 at a cell density of 500 Klett units. Ten microliters of this cell mixture was placed on 1/2 CTT plates with either 1.0 or 0.5% agar. The peninsula-like flares of cells arise from the spot edge as a result of S-motility.
