Fig. 6.

Western blot analysis of LT-B after gastric fluid digestion simulation. (A1 and A2) Ground maize kernels mixed with bacterial LT-B. (B1 and B2) Ground transgenic maize kernels. The samples were treated with pepsin at various length of period and separated on an SDS/18% PAGE and transferred to a 0.45-μm nitrocellulose membrane and probed with rabbit anti-LT-B antibodies. Lanes 1, 5, 10, and 15, 0-min digestion (no digestion control); lanes 2 and 6, 30-s digestion; lanes 3 and 7, 1-min digestion; lanes 4 and 8, 5-min digestion; lanes 9, 11, and 16, 15-min digestion; lanes 12 and 17, 30-min digestion; lanes 13 and 18, 60-min digestion; lane 14, 120-min digestion.