Table 1.
Identification of a male-specific antigen recognized by B6.2.16 CTLs
| Peptide | Sequence | 50max B6.2.16, M |
|---|---|---|
| 1 | PSGKNIGSL | >10−8 |
| 2 | VTLMNPNTL | >10−8 |
| 3 | KCSRNRQYL | 3 × 10−13 |
| 4 | RRFPNSELL | >10−8 |
| 5 | VYLPNIQSL | >10−8 |
| 6 | ELVLNEERI | >10−8 |
| 7 | GQNPNLERI | >10−8 |
The ability of Smcy peptides to sensitize RMA (H-2b) targets to lysis by anti-H-Y B6.2.16 CTLs (15) was tested in 51Cr-release assays and expressed as the concentration of peptide that gave 50% of the maximum specific lysis. The anti-male specificity of the B6.2.16 CTL was demonstrated by measuring the specific lysis of 51Cr-labeled, male (24%) and female (7%) spleen cell blasts from C57BL/6 mice. Similar results were obtained in three other separately performed experiments.