Figure 4.

Decreases in APC GSH levels diminish IFN-γ without diminishing IL-4 production by an OVA-specific DO11.10 transgenic T cell line. Cultures were established with BALB/c APC untreated (hatched bars) or treated (solid bars) with 1.6 mM DEM for 15 min, T cells from a DO11.10 transgenic T cell line, and OVA. T cell proliferation and cytokine production of the culture supernatants were determined. IFN-γ and IL-4 are shown as the mean ± SEM. IFN-γ production was significantly (P < .002) diminished, IL-4 production was slightly enhanced (P < .02), and T cell proliferative responses were unaffected in cultures containing DEM-treated APC (not shown).