Table 2.

IL-12 and IFN-γ are inhibited in cultures containing APC from ethanol-fed mice and DO11.10 OVA-transgenic T cells

Diet		Cytokine production
BALB/c, APC	DO11.10, T cells	IFN-γ, ng/ml	IL-2, ng/ml	IL-12, pg/ml
Liquid ethanol	Solid	68  ±  2.7	39  ±  4.5	<20
Liquid ethanol	Liquid control	25  ±  0.0	33  ±  3.3	<20
Liquid ethanol	Liquid ethanol	35  ±  1.8	51  ±  0.3	<20
Liquid control	Solid	196  ±  0.4	46  ±  2.5	192  ±  7.5
Liquid control	Liquid control	194  ±  1.8	37  ±  0.9	186  ±  0.0
Liquid control	Liquid ethanol	176  ±  11.7	58  ±  1.9	202  ±  22.5
Solid	Solid	235  ±  48.3	59  ±  2.8	298  ±  7.5
Solid	Liquid control	205  ±  11.3	43  ±  1.2	372  ±  22.5
Solid	Liquid ethanol	191  ±  20.3	64  ±  1.5	399  ±  15.0

T cells and APC from unimmunized mice fed liquid ethanol diet, liquid control diet, or standard laboratory chow diet were cocultured with 18 μM of OVA. Cytokine production was measured in the culture supernatants at 48 h (IL-2, IL-12) and 72 h (IFN-γ). Boxed IFN-γ values are significantly lower than liquid control diet (P < 0.0001) and solid diet (P < 0.02) controls. Boxed IL-12 values are significantly lower than liquid control or solid diet (P < 0.0001). In all cultures, IL-4 production was undetectable. IFN-γ production in response to Con A stimulation was not diminished in any cell groups (data not shown).