Table 2.
Diet
|
Cytokine production
|
|||
---|---|---|---|---|
BALB/c, APC | DO11.10, T cells | IFN-γ, ng/ml | IL-2, ng/ml | IL-12, pg/ml |
Liquid ethanol | Solid | 68 ± 2.7 | 39 ± 4.5 | <20 |
Liquid ethanol | Liquid control | 25 ± 0.0 | 33 ± 3.3 | <20 |
Liquid ethanol | Liquid ethanol | 35 ± 1.8 | 51 ± 0.3 | <20 |
Liquid control | Solid | 196 ± 0.4 | 46 ± 2.5 | 192 ± 7.5 |
Liquid control | Liquid control | 194 ± 1.8 | 37 ± 0.9 | 186 ± 0.0 |
Liquid control | Liquid ethanol | 176 ± 11.7 | 58 ± 1.9 | 202 ± 22.5 |
Solid | Solid | 235 ± 48.3 | 59 ± 2.8 | 298 ± 7.5 |
Solid | Liquid control | 205 ± 11.3 | 43 ± 1.2 | 372 ± 22.5 |
Solid | Liquid ethanol | 191 ± 20.3 | 64 ± 1.5 | 399 ± 15.0 |
T cells and APC from unimmunized mice fed liquid ethanol diet, liquid control diet, or standard laboratory chow diet were cocultured with 18 μM of OVA. Cytokine production was measured in the culture supernatants at 48 h (IL-2, IL-12) and 72 h (IFN-γ). Boxed IFN-γ values are significantly lower than liquid control diet (P < 0.0001) and solid diet (P < 0.02) controls. Boxed IL-12 values are significantly lower than liquid control or solid diet (P < 0.0001). In all cultures, IL-4 production was undetectable. IFN-γ production in response to Con A stimulation was not diminished in any cell groups (data not shown).