Figure 1.
TR1–41-, TR42–55-, and thrombin-induced platelet activation. The indicated concentrations of TR1–41-, TR42–55-, and thrombin, or control buffer were incubated (22°C, 10 min) with diluted whole blood and then fixed. The platelet surface binding of the mAbs S12 (directed against P-selectin, A), PAC1 (directed against the activated GPIIb-IIIa complex, B) and F26 (directed against fibrinogen bound to the GPIIb-IIIa complex, C) were determined by whole-blood flow cytometry. Binding is expressed as a percent of the binding with maximal thrombin (2 units/ml). Data are mean ± SEM. n = 6.