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. 1998 Mar 17;95(6):3111–3116. doi: 10.1073/pnas.95.6.3111

Figure 3.

Figure 3

(A) Infection of activated mature CD8+ T cells by HIV-1. Seven-day-old PHA-activated CD4-depleted PBMC were infected with HIV-1 IIIB in the absence or the presence of OKT4A or anti-CD28. At various times after infection, cell-free supernatants were collected and stored until HIV-1 p24 antigen determination. Results (mean ± SD) were calculated from triplicate cultures and are representative of two separate experiments. (B) Disappearance of CD4+CD8+ T cells following HIV-1 infection. Twelve days post-HIV-1 infection of activated CD8+ T cells, cells were stained for both CD8 and CD4. Cells from uninfected cultures were simultaneously analyzed for CD4 and CD8 markers. (C) Intracellular detection of HIV p24 in CD8+ T cells. Six-day-old PHA-activated CD4-depleted PBMC were treated with HIV-1 for 5 days and analyzed for cell surface CD8 and intracellular HIV-1 p24 antigen expression.