Skip to main content
. 1998 Mar 17;95(6):3146–3151. doi: 10.1073/pnas.95.6.3146

Figure 1.

Figure 1

Purification and heat-modifiable mobility of PapC and FimD. (A) Coomassie blue-stained SDS/PAGE showing W3110II5/pMJ3 inner (lane IM) and outer (lanes OM) membrane fractions isolated during PapC purification, and PapC and FimD after purification from the OM by nickel chromatography. OM, PapC, and FimD samples were heated to 42°C or 95°C in sample buffer for 5 min before loading. IM was treated at 95°C. (B) Anti-PapC immunoblot of OM from strains W3110II5/pMON6235 (PapC−) or W3110II5/pMJ3 (PapC+). Samples were heated to 42°C or 95°C in sample buffer for 5 min before SDS/PAGE and transfer to a poly(vinylidene difluoride) membrane.