Figure 4.

Oligomeric state of PapC determined by Blue native PAGE. Coomassie blue G (0.05%) was added to purified PapC, which was loaded directly (lane 1) or heated to 95°C for 5 min in the presence of 1.0% SDS and 2.5% 2-mercaptoethanol (lane 2) before loading. Protein standards (lane 3) were treated as in lane 1. The standards were thyroglobulin (669 kDa), ferritin (440 kDa), catalase (232 kDa), lactate dehydrogenase (140 kDa), and albumin (67 kDa). The band of slowest mobility in lane 1 is consistent with a hexameric PapC complex.