Figure 2.

Restriction enzyme analysis of the G protein genes of CVS-24, CVS-N2c, and CVS-B2c. Genomic rabies RNA was isolated from virions and a 820-bp fragment of the G protein gene was amplified by RT-PCR. (A) Digestion of the PCR product obtained from CVS-N2c with NsiI results in a 410-bp fragment, whereas the corresponding CVS-B2c PCR product is not cleaved. (B) Restriction enzyme analysis of the G protein genes of CVS-24 passaged in mouse brain (CVS-24) and after two consecutive passages in NA cells (N2) or BHK cells (B2). (C) Restriction enzyme analysis of the G protein genes of viruses obtained three parallel passaging experiments (passages 1–3).