Figure 3.

Selection for CVS-N2c of and CVS-B2c in neonatal and adult mice. Neonatal and adult mice were intracranially infected with 10³ FFU of mixtures containing different ratios of CVS-N2c (N2c) and CVS-B2c (B2c). Brains were harvested from neonatal mice 4 days after infection and from adult mice 5 days after infection, total RNA was isolated from the brains, and 3 μg of total RNA was subjected to RT-PCR/restriction enzyme analysis as described in Fig. 2. After gel electrophoresis, the DNA bands were quantitated by densitometry using the nih 1.58 image program.