Figure 5.
Rabies virus RNA replication in muscle tissue at the side of inoculation. Approximately 105 FFU of CVS-N2c (N2C) or CVS-B2c (B2c) was inoculated into the masseter muscle of adult mice, and at 24 and 48 hr after infection, muscle tissue was harvested from three mice (lanes 1–3), total RNA was isolated from the muscle tissue and 2.5 μg of total RNA was subjected to the RT-PCR. Rabies virus nucleoprotein mRNA was then selectively reverse-transcribed with an antisense primer, subjected to PCR amplification, and analyzed by gel electrophoresis and Southern blotting. G3PDH mRNA was RT-PCR-amplified from each RNA sample to serve as a loading control.