Figure 3.

Analysis of the cloned zebrafish β3 subunit gene. Presentation and labeling as in Figure 1. A) Alignment of cloned human, mouse, rat, and zebrafish β3 amino acid sequences. INT = putative internalization sequence. B) 5' and 3' RLM-RACE PCR and RT-PCR identified a single transcript expressed from the zβ3 locus on zebrafish chromosome 15 (Ensembl). zβ3 has six exons. C) All zβ3 splice sites adhere to GT-AG consensus sequences. D) Schematic diagram of the β3 protein.