Figure 8.
Transient expression of human Cyclin T1 in primary rat T-cells boosts early HIV gene expression. (A) Sequence alignment of amino acids 250–290 of human, rat and mouse Cyclin T1. * depicts the critical amino acid position 261. (B) Proviral pHIV-1NL4-3 GFP or pHIV-2ROD-A GFP constructs were co-transfected with increasing amounts of an expression vector encoding for either human Cyclin T1 (phCyclin T1) or no transgene (pcDNA3.1), and cells were analyzed for GFP expression 20 h later by flow cytometry.