Skip to main content
. 1998 Mar 17;95(6):3157–3161. doi: 10.1073/pnas.95.6.3157

Figure 1.

Figure 1

(a) Electrophoretic analysis of RNA associated with FLAG-Rne-containing degradosomes. RNA extracted from degradosomes (lane C), purified ribosomes (lane R), or total lysates of E. coli (lane T) was separated on 5% denaturing polyacrylamide gels and stained with ethidium bromide. Positions of 23S, 16S, and 5S rRNAs and tRNAs are shown. (b) Electrophoretic analysis of RNA associated with immunoaffinity-purified native Rne complexes. RNA extracted from FLAG-Rne-containing degradosomes and total lysates of E. coli (lanes C and T, respectively) from purified native Rne complexes (lane A) were analyzed as described for a. Lane P shows a background RNA bound to protein A-Sepharose coupled with the preimmune serum antibodies. (c) Clones of cDNA derived from rRNA fragments associated with FLAG-Rne degradosome. Inserts of cDNA clones aligned with previously determined sequences of 16S and 23S rRNA. Arrowheads indicate the positions of oligonucleotide primers.