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. 1998 Mar 17;95(6):3157–3161. doi: 10.1073/pnas.95.6.3157

Figure 2.

Figure 2

Northern blot analysis of rRNA associated with degradosomes. RNA extracted from FLAG-Rne-containing degradosomes (lane C), from ribosomes (lane R), or total E. coli lysate (lane T) was loaded onto 5% denaturing polyacrylamide gel and resolved by electrophoresis. RNA was transferred to a membrane and probed with uniformly labelled RNAs complementary to 16S (a), 23S (b), and 16S and 23S (c and e). (d) Results of Northern blot analysis where 9S antisense riboprobe was used. (e) Results of Northern blot analysis of RNA associated with immunoaffinity-purified native Rne complexes (lane A; see Fig. 1b). Positions of 23S, 16S, and 5S rRNAs are shown.