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. 1998 Mar 17;95(6):3157–3161. doi: 10.1073/pnas.95.6.3157

Figure 5.

Figure 5

Cleavage of RNA components by FLAG-Rne containing degradosomes. (a) RNA extracted from degradosome was incubated in RNase E reaction buffer supplemented with ATP and phosphate for 15 min at 37°C without (lane 2) and for 5 and 15 min with degradosome (lanes 3 and 4). E. coli tRNA was added to reaction mixture as an internal negative control for cleavage (lanes 2–4). Lane 1 contains total E. coli RNA. (b) Northern blot analysis of cleavage products. RNA probes complementary to 16S and 23S rRNA were used to detect fragmented rRNAs.