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. Author manuscript; available in PMC: 2007 Sep 25.
Published in final edited form as: Endocrinology. 2006 Oct 12;148(1):241–251. doi: 10.1210/en.2006-0692

Figure 7.

Figure 7

Prolonged IL-1β treatment induces ERK1/2 and p38MAP Kinases phosphorylation. 3T3-L1 adipocytes were treated without or with IL-1β (20 ng/ml) for 48 hours. Proteins from cell lysates were blotted using anti-phospho ERK1/2 (α-P-ERK) or anti-phospho-p38MAPK (α-P-p38) antibodies. The membranes were then stripped and probed using anti-ERK1/2 (α-ERK1/2) or anti-p38 (α-p38) antibodies. Representative immunoblots and means ± SEM of four independent experiments are shown. Results are expressed as fold phosphorylation over basal set to 1 in untreated cells (* p < 0.05).