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. 2007 Sep 7;3(9):e128. doi: 10.1371/journal.ppat.0030128

Figure 3. Extra- and Intracellular Siderophore Production of A. fumigatus wt, ΔsidA, ΔsidC, ΔsidD, ΔsidF, and ΔsidG .

Figure 3

(A) Representative HPLC analysis of culture supernatants, cell extracts, and conidial extracts of the wt. Units are given in milli absorption units (mAu).

(B) Quantification of siderophore production of ΔsidA, ΔsidC, ΔsidD, ΔsidF, and ΔsidG normalized to that of the wt after 24 h of growth in iron-depleted conditions. For HPLC analysis of the conidial siderophore, the wt and mutant strains were grown for 5 d either with 0.5 mM FeSO4, 10 μM FC (FC), or 10 μM TAFC (TAFC), respectively, as iron source. 1The FSC and FC contents in wt supernatant represented 12.0±3.1% and 1.5±3.1% of the TAFC content, respectively. The data represent the means ± standard deviations of results from three independent experiments. ND, not detected; na, not analyzed.