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D. mauritiana P[w +]-marked chromosome segments were introgressed into a D. sechellia w background via 15 generations of backcrossing (A). The P[w +] insertion carries a mini-white gene that acts as a partially dominant visible eye-color marker for selecting heterozygous females for each backcross. Introgressions were made homozygous on the autosomes (B) and the X chromosome (C), and tested for postzygotic hybrid incompatibilities.
