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. Author manuscript; available in PMC: 2008 Jul 27.
Published in final edited form as: Cell. 2007 Jul 27;130(2):335–347. doi: 10.1016/j.cell.2007.05.043

Figure 3. UgtP inhibits FtsZ assembly in vitro.

Figure 3

(A–D) Thio-UgtP blocks FtsZ assembly in a 90° angle light scattering assay. FtsZ is at 6 μM in all reactions. Unless noted, all reactions contain 1 mM UDP-Glc and 1 mM GTP. Error bars = standard deviations. FtsZ assembly in (B–D) is expressed as the assembly of FtsZ in the presence of the component indicated relative to the absence of the component indicated. (A) A representative light scattering trace showing FtsZ assembly ± 2 μM Thio-UgtP. AU = arbitrary units. Arrow indicates addition of 1 mM GTP. (B) Concentration-dependent inhibition of FtsZ assembly by UgtP. The ratio of Thio-UgtP to FtsZ is shown beneath each bar. Purified Thio-UgtP is shown inset. (C) Inhibition of FtsZ assembly by 2 μM Thio-UgtP is only mildly stimulated by 1mM UDP-Glc. (D) FtsZ assembly in the presence of either 6 μM Thio-UgtP, Thioredoxin-his, or BSA. (E–H) Electron micrographs of FtsZ and UgtP. FtsZ is 2 μM and Thio-UgtP is 1 μM. All reactions were conducted in the presence of 1 mM UDP-Glc and 1 mM GTP. Bar = 100 nm for (E–G) and 50 nm for (H). (E) FtsZ alone. Arrow indicates bundle of polymers running the length of the micrograph. (F) FtsZ assembled in the presence of Thio-UgtP. Only short polymers are present and little bundling is observed. (G) Thio-UgtP oligomers formed in the absence of FtsZ. The selected region in (G) is shown at 2-fold greater magnification in (H).