Fig 1.

 Cloning and sequencing of Artemia p8. (A) RNA from diapause destined embryos was extended by 3′- and 5′-RACE. DNA products were electrophoresed in agarose gels and stained with Gel Star. Lane 1: 3′-RACE lacking cDNA; lane 2: 3′-RACE; lane 3: 5′-RACE lacking cDNA; lane 4: 5′-RACE; M, size markers in bp. (B) The complete p8 cDNA and deduced amino acid sequences. Boxed C and A, transcription start sites; boxed ATG, translation start site; boxed TGA, stop codon; boxed AATAAA, polyadenylation signal; shaded ATTTA, sequences indicative of rapidly degraded mRNAs