Fig 3.

 Artemia p8 gene. (A) Digested Artemia genomic DNA was electrophoresed in agarose, transferred to nylon membranes, and probed with p8 cDNA. Lanes 1–5: loaded with DNA digested, respectively, with XhoI, PstI, SalI, BamHI and HindIII. Size in kbp is indicated on the left. (B) Upstream sequence of the p8 gene obtained by LA polymerase chain reaction (PCR) in vitro cloning. Boxed TATAA, TATA box; bold C with arrow, transcription start site; several putative cis-acting regulatory sites are underlined and labeled