Figure 2.

Failure of LIFR^−/− neural cells to express GFAP. Neuroepithelial cells from E12 forebrain were plated in vitro at a density of 2.5 × 10⁴ per 200 mm² into multiwell plates (Falcon 3047) and cultured for 20 days in the presence of serum. Cultures then were stained by immunoperoxidase for the presence of GFAP. The LIFR^+/+ cultures contained large numbers of GFAP-positive cells (A and C) whereas the LIFR^−/− cultures (B and C) contained few if any (<2 cells per well in every case). The data shown in C are the mean and SEM obtained from three separate experiments, each with three replicates.