Figure 2.

Amylase release in pancreatic acini. Abilities of CCK-8 (Left), BN (Center), and carbachol (Right) to stimulate amylase release from dispersed pancreatic acini prepared from GRP-R-targeted (−/Y) male mice (solid symbols) or control (+/Y) male littermates (open symbols). Dispersed acini were incubated with the indicated concentrations of secretagogues for 30 min at 37°C. Results are expressed as the percentage of total cellular amylase activity in the cells before the incubation that was released into the extracellular medium during the incubation and are the means ± SEM of three experiments performed in triplicate.