TABLE 1.

Primers for Fundulus CYP1C1 Cloning and Real-Time PCR

Oligo	Primer Sequence 5′–3′	Location	Function
1	AGTTTGGTTGATGTCTTGCC	897–916	1C1 forward
2	GTCAAAGGCTTGAGGGTCCT	1459–1478	1C1 reverse
3	CACCTCAGGGGGTCGTGATTGACAG	1432–1456	1C1 5′RACE reverse
4	GGGGGTCGTGATTGACAGACCACTG	1425–1449	1C1 5′RACE reverse
5	CGCTTCACCAGCTTTGTCCCATTCA	1323–1347	1C1 3′RACE forward
6	CCCAAGGACACGGTGGTCTTCATCA	1398–1422	1C1 3′RACE forward
7	CTTAAGCCTTAAATCTTGATGATAGAC	1–27	1C1 forward for full-length
8	CAGTATATAAAGGCTCAGTGGGACTC	2698–2723	1C1 reverse for full-length
9	AACACTAAAGCAACAACTCCAGATT	2672–2696	1C1 reverse for full-length
10	TCTGGACGCCTTCATCTACGA	1295–1315	1C1 real-time forward
11	GTGACGTCCGATGTGGTTGA	1357–1378	1C1 real-time reverse
12	CGAAGATTTTGTCCAGGTGACA	816–837	1A real-time forward
13	CATTGACTTGTTGGGCAGAAACT	874–897	1A real-time reverse
14	TGGTTAATTCCGATAACGAACGA	1319–1341	18S real-time forward
15	CGCCACTTGTCCCTCTAAGAA	1395–1415	18S real-time reverse