TABLE 2.

CYP1 mRNA Expression in Adult Fundulus Exposed 15 Days to Ethanol Control or 10 μg/l BaP

	Males			Females
	Fold BAP inductiona		Control isoformb	Fold BAP inductiona		Control isoformb
	CYP1A	CYP1C	Fold abundance	CYP1A	CYP1C	Fold abundance
Heart	18.9 ± 9.70	16.8 ± 9.26	14.6 ± 2.77 more 1A versus 1C1	27.9 ± 10.6	30.5 ± 21.8	14.6 ± 3.64 more 1A versus 1C1
Gill	14.2 ± 2.16	4.47 ± 0.457	3.94 ± 0.478 more 1A versus 1C1	13.8 ± 1.47	6.60 ± 1.74	3.05 ± 0.447 more 1A versus 1C1
GI	6.75 ± 3.77	1.20 ± 0.14	8.05 ± 1.23 more 1A versus 1C1	1.59 ± 0.46	1.03 ± 0.17	5.12 ± 1.46 more 1A versus 1C1
Liver	1.43 ± 0.25	4.65 ± 1.26	27.4 ± 2.78 more 1A versus 1C1	1.70 ± 0.28	5.85 ± 1.73	33.1 ± 9.76 more 1A versus 1C1
Brain	23.6 ± 15.7	1.14 ± 0.08	8.63 ± 1.21 more 1C1 versus 1A	37.6 ± 20.5	1.13 ± 0.32	16.7 ± 1.63 more 1C1 versus 1A
Eye	12.3 ± 4.70	1.08 ± 0.16	6.27 ± 0.692 more 1C1 versus 1A	28.2 ± 6.52	2.06 ± 0.422	3.39 ± 0.870 more 1C1 versus 1A
Gonad	25.1 ± 11.2	0.79 ± 0.40	108 ± 24.6 more 1C1 versus 1A	9.71 ± 2.96	1.26 ± 0.39	65.3 ± 20.7 more 1C1 versus 1A
Spleen	1.61 ± 0.28	1.90 ± 0.86	39.2 ± 11.9 more 1C1 versus 1A	12.4 ± 2.59	2.15 ± 0.75	80.8 ± 22.6 more 1C1 versus 1A
Kidney	2.90 ± 0.653	1.62 ± 0.37	1.14 ± 0.13 more 1C1 versus 1A	3.00 ± 0.395	2.30 ± 0.37	1.07 ± 0.20 more 1C1 versus 1A

^aCalculated by ΔΔC_T approach. Data normalized to 18S expression and reported as fold induction relative to controls (n = 3–5).

^bCalculated by ΔΔC_T approach. CYP1A and CYP1C1 expression in control animals was normalized to 18S expression and is shown as relative fold abundance.

Numbers in bold are significantly different, ANOVA p < 0.05, as analyzed on 18S corrected raw data.

Gray-shaded rows represent tissues where CYP1C1 expression was constitutively higher than CYP1A expression.