Fig. 3. Enzymatic activities of PBCV-1 AIH and CPA.
The release of ammonia (A) as well as the turnover of the substrates to the products (B) were analyzed. Agmatine (1 mM) was incubated with PBCV-1 AIH (20 μg) for 90 min, then 15 μg of PBCV-1 CPA was added. C, C’: controls with heat denatured enzyme after 10 and 240 min incubation, respectively; M: standard mixture of 2 mM each of agmatine (Agm), N-carbamoylputrescine (N-CP) and putrescine (Put).