Table 1.
Oligonucleotide primers used for site-directed mutagenesis
| Mutation | Direction | Nucleotide sequence of primer1 |
|---|---|---|
| N100A | Forward | 5′-TGAATGTCGCTGTGAAAGGTACCCAGC-3′ |
| Reverse | 5′-CTTTCACAGCGACATTCATGATAGACTCT-3′ | |
| N100S | Forward | 5′-GAATGTCAGTGTGAAAGGTACCCAGC-3′ |
| Reverse | 5′-CTTTCACACTGACATTCATGATAGACTC-3′ | |
| H232A | Forward | 5′-TGGGCCGCCATTCTGGCCTTGAGG-3′ |
| Reverse | 5′-CAGAATGGCGGCCCAGGCCACATTGC-3′ | |
| S322A | Forward | 5′-CACATTGGCAAATAGCGTATTCAC-3′ |
| Reverse | 5′-CGCTATTTGCCAATGTGACTATGTG-3′ | |
| N323L | Forward | 5′-CATTGTCACTTAGCGTATTCACCTTCTC-3′ |
| Reverse | 5′-TACGCTAAGTGACAATGTGACTATGTGG-3′ | |
| E126L | Forward | 5′-AGCATACTGGTAGCCGGGCCCAACTC-3′ |
| Reverse | 5′-GGCTACCAGTATGCTACTGGTGTAG-3′ |
1
The mutated codons are underlined.