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Confocal laser microscope images of Reelin mRNA detected by in situ hybridization using a biotinylated oligoprobe (base pairs 1264–1311) visualized with fluorescein (green) (Top), and GAD67 immunohistochemistry visualized with rhodamine (red) (Middle), in a hippocampal CR cell at P0. (Bottom) Overlay of Top and Middle showing that Reelin mRNA colocalizes with GAD67 (yellow). (×4,000.)
