Figure 5 Sequential 5 μm section immunohistochemistry with anti‐human defensin (HD)‐5 antibody (A, B), anti‐lysozyme antibody (C, D) and anti‐intestinal trefoil factor antibody (E, F) seen at low (A, C, E) and high (B, D, F) magnification. An intermediate cell on the villus tip (large arrow) is seen to express HD‐5 and lysozyme, whereas an intermediate cell towards the villus base expresses HD‐5 but not lysozyme (small arrow). The lack of Paneth cell staining using the rabbit polyclonal anti‐intestinal trefoil factor antibody (E, F) in these sequential sections also serves as a control for the HD‐5 and lysozyme polyclonal antisera.