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. 1998 Mar 17;95(6):3251–3256. doi: 10.1073/pnas.95.6.3251

Figure 3.

Figure 3

Sympathetic neuron survival is stimulated by sIL-6R and endogenous IL-6. (A) Silver-stained protein gel after SDS/PAGE of supernatants of transfected cells (lane 1) and purified sIL-6R (lane 2). (B) Western blot analysis of the gel shown in A, using a rabbit antiserum raised against recombinant rat sIL-6R. (C) Neurons were plated in medium (control), 100 ng/ml NGF, 10 ng/ml H-IL-6, 100 ng/ml human IL-6 + 1 mg/ml human sIL-6R, or 10 ng/ml rat sIL-6R. Six days after treatment, neuronal survival was determined by the MTT assay.