Figure 2.

Increased H₂O₂-Induced Cell Death in PLDδ-Null Arabidopsis Protoplasts.

(A) Protoplast death as a function of H₂O₂ concentration. WT, wild type.

(B) H₂O₂ (1 mM)-induced cell death as a function of incubation time.

(C) Complementation of the PLDδ-null mutant with the wild-type PLDδ gene and restoration of the mutant response to H₂O₂ to wild type. The inset shows an immunoblot analysis of proteins extracted from wild-type, PLDδ-complemented (PLDδ-C), and PLDδ-null plants with PLDδ-specific antibody. The double asterisks indicate that the mean value is significantly different from that of wild-type protoplasts (P < 0.01).

Dead cells were counted 24 h after H₂O₂ treatments and are expressed as a percentage of total cells.